Automated NEBNext Ultra Directional RNA Library Prep Kit
Webinar from Daniel Jasinski covers RNA nanotechnology in cancer therapy. RNA/RNA-based nanoparticle purification by ultracentrifugation including current technologies, different methods of ultracentrifugation, why large quantities of RNA are needed, methods for large-scale RNA purification.
Part 2 in three-part series covering automated NGS workflows. This session addresses automated DNA & RNA sequencing methods including whole genome sequencing and Illumina products (TrueSeq DNA PCR-Free, Nano DNA); targeted DNA sequencing including Agilent HaloPlex; the transcriptome and automated total RNA and mRNA sequencing and library construction/QC, including a results overview.
Michaela Bowden (CEMOP Associate Director, Dana Farber Cancer Institute) presents the utility of Biomek automation (library prep, etc.) and using biopsied/low RNA-input (FFPE) samples for clinically relevant results. Includes overview of the RNA-Seq workflow and Illumina's Script-Seq Complete, TruSeq Stranded mRNA kits.