Purificación de PCR
El sistema Agencourt AMPure XP es un sistema de purificación de PCR sumamente eficiente y de automatización sencilla que ofrece ADN de calidad superior sin arrastre de sal. Dado que no requiere de centrifugación ni de filtración, Agencourt AMPure XP puede usarse fácilmente en formatos de 96 o 384 pocillos manuales y automatizados.
Choose a PCR Model
AMPure XP Workflow
|Application Uses||Purification & Clean-up, PCR Purification, DNA Purification, Sequencing, NGS Clean-up, PCR clean-up|
|Starting Sample Material||DNA|
|Item Specifications Referenced||A63882|
Peffers, M. J., Liu, X., & Clegg, P. D. (2014, March 8). Transcriptomic profiling of cartilage ageing. Genomics Data, 2, 27-28. doi:10.1016/j.gdata.2014.03.001
- Used to clean up cDNA
Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-5
- Used to clean up barcoded PCR reactions during Library preparation
Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.
Don't Lose Critical Data
When so much more has been invested in your research, AMPure XP is the only choice for purification and clean-up steps. Loss of yield during this critical step leads to loss of discovery in your research.
Relative costs of the different steps required to perform various NGS applications. Steps include Extraction, Library Construction, Library Enrichment, Clean-up, and Sequencing. Costs were calculated based on average list price of commercially available kits and reagents in 2017. Clean-up efficiencies were calculated by determining the total DNA yield by Picogreen Assay after performing a clean-up procedure on a known amount of DNA. The percent yield relative to AMPure XP performance was then used to calculate the impact of efficiency on various commercially available library construction methods and a change in purification reagent.