Viral RNA Extraction

Reagent Kits: RNAdvance Viral or RNAdvance Viral XP

Extract high-quality RNA from Saliva or Swab Transport Media samples using SPRI paramagnetic bead-based technology. Our viral RNA extraction kits are available for your unique input sample types and collection devices. RNAdvance Viral XP offers higher throughput capabilities and the RNAdvance Viral kit is optimal for extracting from a variety of challenging sample inputs.

  • Produces high-integrity viral RNA for use with PCR-based applications for infectious disease and pathogen research
  • Performance Limit of Detection (LoD) demonstrated at 1 copy/uL for viral RNA
  • Automation-ready, viral extraction quickly adapted to liquid handling platforms

Technical Documents:

For research use, not intended for diagnostic purposes.

 

Resources + Tools
Performance Automated Workflow Formación Customer Interview Testimonial White Paper

Explore Viral Models

Pasos del flujo de trabajo de RNAdvance Blood

Genómica RNAdvance Blood Flujo de trabajo

Características del producto

Descripción general
RNAdvance Blood aísla ARN de diversos tubos de preservación de ARN (p. ej., tubos de ARN de sangre PAXgene).

ARN de alta calidad
El kit de aislamiento RNAdvance Blood purifica el ARN intacto que es apto para aplicaciones basadas en PCR posteriores.

Flexible y escalable
La combinación de la química de RNAdvance Blood con nuestra estación de trabajo Biomek ofrece una solución autónoma y de alto rendimiento.

Especificaciones del producto

Application Uses Research Use, RNA Isolation, RNA Extraction, Nucleic Acid Sample Prep, PCR
Format Liquid
Starting Sample Material Saliva and Swab in Universal Transport Media
Automated Available Yes
Item Specifications Referenced C63510

Citas

Beineke, P., Fitch, K., Tao, H., Elashoff, M. R., Rosenberg, S., Kraus, W. E., . . . PREDICT Investigators. (2012, December 3). A whole blood gene expression-based signature for smoking status. BMC Medical Genomics, 5(58). doi:10.1186/1755-8794-5-58

  • Se utiliza para extraer ARN de muestras de sangre recogidas en PAXgene

Allaire, N. E., Bushnell, S. E., Bienkowska, J., Brock, G., & Carulli, J. (2013, January 5). Optimization of a high-throughput whole blood expression profiling methodology and its application to assess the pharmacodynamics of interferon (IFN) beta-1a or polyethylene glycol-conjugated IFN beta-1a in healthy clinical trial subjects. BMC Research Notes, 6(8). doi:10.1186/1756-0500-6-8

  • Se utiliza para extraer ARN de muestras de sangre recogidas en PAXgene

Disclaimer (Aviso de exención de responsabilidad): Beckman Coulter no ofrece garantías de ningún tipo, expresas o implícitas, con respecto a este protocolo, incluyendo, entre otras, las garantías de idoneidad para un propósito particular o de comerciabilidad o de que el protocolo no sea infringente. Todas las garantías están expresamente rechazadas. Su uso del método es exclusivamente bajo su propio riesgo, sin recurrir a Beckman Coulter. No está destinado ni se ha validado para su uso en el diagnóstico de enfermedades u otras afecciones. Este protocolo es para demostración solamente y no está validado por Beckman Coulter.

Content and Resources

High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB® Luna® Reagents Application Note: We show that NEB Luna qPCR reagents offer strong performance in high-throughput qPCR applications and are readily compatible with automated reaction setup, here using the Beckman Echo 525 acoustic liquid handler.
Characterization of the Beckman Coulter's RNAdvance Viral XP RNA Extraction Kit using AccuPlex™ SARS–CoV–2 Reference Material Kit White Paper: iRepertoire performed analytical performance assays and specimen assays to demonstrate that the RNAdvance Viral XP extraction kit has a limit of detection of RNA (LoD) of 1 copy of RNA, specifically tested with AccuPlex™ SARS–CoV–2 Reference Material Kit, per microliter of sample.
Inactivation of COVID-19 disease virus SARS-CoV-2 with Beckman Coulter viral RNA extraction lysis buffers White Paper: We conducted experiments to assess the virus inactivation status after treatment with lysis buffer (LBF) from RNAdvance Viral or RNAdvance Viral XP extraction kits.
Infectious Disease-based Pandemic Monitoring using Distributed Sewer Water Samples Poster: In this proof of principle study, we demonstrate RNAdvance Viral RNA extraction method that's compatible with different wastewater concentration methods.
Robust and High-Throughput SARS-CoV-2 Viral RNA Detection, Research, and Sequencing using RNAdvance Viral and the OT-2 Platform Application Note: We present analytical data showing the SARS-CoV-2 limit of detection (LoD) quantified by qRT-PCR and sequence alignment data that establishes proof of principle for automated sequencing sample and library preparation for possible genetic identification of mutated strains.

Documentos técnicos